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Amplitude Ventures
Amplitude Ventures

Scientist I, Preclinical Sciences

Notch Therapeutics

Notch Therapeutics

Seattle, WA, USA
Posted on Thursday, May 23, 2024

Role Summary

We are seeking a highly motivated Scientist I in the Preclinical Sciences team to support our iPSC and primary immune cell engineering initiatives. This position will be responsible for implementing and optimizing a cell engineering workflow from designing and screening appropriate gene editing tools, identifying delivery methods, building medium/high throughput screening in primary immune cells, and the assessment and quantification of on and off-target editing. This role will also play a vital part in supporting Notch’s cutting-edge innovation of therapeutic cell design through molecular cloning and screening of functional payloads, and -omics-based characterization of engineered cells. Direct experience in molecular biology, genomics, and the ability to independently design and execute complex cell engineering experiments are essential.

Key responsibilities:

Support advancement of our genome editing pipeline and platform

  • Screen and characterize gRNAs for gene KO/KI efforts
  • Design, clone, and evaluate nucleic acid reagents for CRISPR-mediated site-specific insertion of transgenes
  • Establish a medium-high throughput genome engineering workflow for gene KO and KI in primary immune cells
  • Carry out appropriate methods for measuring genome editing efficiencies and characterizing repair outcomes for on and off-target editing

Design and implement genomic and transcriptomic NGS assays to characterize engineered cells

  • Execute library design, preparation, QC, and troubleshooting
  • Manage relationships with CROs
  • Collaborate with the bioinformatics team to facilitate efficient data processing and analysis

Participate in the design, rapid construction, and efficient screening of functional payloads for engineered immune cells

  • Collaborate with cancer biology and cell engineering teams to progress designs from conception through various stages of evaluation

Maintain project timeline and report progress regularly to the project team

  • Maintain excellent records of experiments in lab notebooks in a timely manner

Other responsibilities as required

  • Present data at group and company meetings and externally as required
  • Participate in external collaborations with partners and CROs
  • Support lab operations and equipment purchasing/ vendor selection

Role Requirements and Qualifications

  • PhD in molecular biology, cell biology, bioengineering, chemical engineering or a closely related discipline with 2+ years of postgraduate work experience in a biotech or industry setting or master’s degree with equivalent experience in a biotech/pharma setting
  • Extensive knowledge of gene-editing technologies and methods for both KO and KI
  • Proficiency in construct design, codon optimization, and molecular cloning techniques
  • Experience in characterizing cells at the genetic and transcriptional levels using -omics approaches as well as ddPCR and RT-PCR
  • Expertise in execution of NGS wet-lab methods and data analysis using python or R
  • Experience with single cell sequencing and 10X platform
  • Working knowledge of T-cell culture, activation/expansion, and functional assays
  • Experience with flow cytometry for cell analysis and sorting
  • Ability to effectively prioritize, plan, and execute next steps based on experimental results in a fast-paced research environment.
  • Team-oriented, can-do attitude. Motivated to contribute and to learn new techniques.
  • Strong organizational, record-keeping and communication skills, both verbal and written

Desired Characteristics and Demonstrated Skills

  • Immunology background would be an advantage
  • Experience using long-read sequencing technologies (e.g. nanopore, PacBio)
  • Experience working with iPSCs would be an advantage
  • Demonstrated ability and keen interest in modelling Notch’s Core Values: Collaborative, Motivated, Ingenious

Salary Range: $106,000 - $138,000


  • Paid Vacation and Sick time
  • Hybrid Work Framework
  • Healthcare plans
  • 401k/RRSP plan
  • Commuting Financial Assistance
  • Home Office WIFI Support
  • Employee assistance programs (EAPs)
  • Internal learning & development opportunities

Notch Therapeutics Inc. is an equal opportunity employer. All qualified applications will receive consideration for employment without regard to race, age, gender identity, sexual orientation, color, religion, sex, marital status, national origin, protected veteran status, disability status, or any other status protected by federal, provincial or local law.

Requests for accommodation can be made at any stage of the recruitment process providing the applicant has met the Bona-fide requirements for the open position. Applicants need to make their requirements known when contacted.